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Fig. 3 | Journal of Ovarian Research

Fig. 3

From: L-DOPA in the hu man ovarian follicular fluid acts as an antioxidant factor on granulosa cells

Fig. 3

Production of H2O2 and ROS in cultured human GCs. a Measurement of H2O2 using amplex red shows generation of H2O2 occurring over 2 h. Values are shown as mean ± S.E.M of n = 3 experiments. b L-DOPA (200 nM) significantly blocks H2O2 (1 mM) dependent DCF fluorescence intensity (P < 0.05, ANOVA, Newman-Keuls). Values are shown as mean ± S.E.M. of n = 3 independent preparations of cells from two to five patients each. Note that shown values are resulting from endpoint measurements after 2 h

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