Fig. 3

Immunofluorescence analysis. Immunofluorescence visualization of: (a) MRP2 protein in A2780, A2780CR1 and A2780CR2 cell lines; (b) P-gp protein in A2780, A2780DR1, A2780DR2, A2780PR1, A2780PR2 and A2780TR2 cell lines; (c) BCRP protein in A2780, A2780TR1 and A2780TR2 cell lines; (d) LRP/MVP protein in A2780, A2780PR1 and A2780PR2 cell lines. All antigens were detected using respected primary antibodies and corresponding MFP488-conjugated secondary antibodies (green). To visualize the cell nuclei, the cells were mounted with a DAPI-containing mounting medium (blue). Objective x40