Fig. 3

Regulation of JAK3 mRNA and protein partners during follicular development. Panels a), b), and c) Total RNA extracts of GC from SF, DF, OF, and CL were used to analyze LEPROTL1, INHBA and CDKN1B expression by real time RT-qPCR with GAPDH as reference gene. LEPROTL1 mRNA expression was significantly increased in the CL as compared to SF (a) while there were no differences among the SF, DF and OF groups. Expression of INHBA was significantly greater in DF as compared to SF, OF and CL (b); expression of INHBA was also greater in SF than in OF and CL (b). For CDKN1B, mRNA expression was significantly greater in the CL as compared to SF, DF and OF (c)