Fig. 5

Phosphorylation assays of STAT3 and STAT5 by JAK3 in endometrial cells. Endometrial cells were put into culture and transfected or not with JAK3. Cells were then treated with 100 μM of JANEX-1 for 4, 8 and 24 h while control cells did not receive JANEX-1 treatment. Cells were collected using the M-PER buffer and protein extracts were subjected to western blot analyses. A) JANEX-1 reduced the phosphorylation of STAT3 after 8 h of treatment and more so after 24 h, while transfection with JAK3 prior to JANEX-1 treatment increased the amount of pSTAT3. B) JANEX-1 reduced the amount of pSTAT5 in endometrial cells not transfected with JAK3 while transfection with JAK3 considerably reduced the amount of pSTAT5 as compared to non-transfected cells