Differential regulation of Janus kinase 3 (JAK3) in bovine preovulatory follicles and identification of JAK3 interacting proteins in granulosa cells

Table 1 List of confirmed JAK3 partners in granulosa cells

Genes	Accession #^a	Freq.	Ident. (%)	E-value	Description
INHBA	NM_174363.2	4	100	0.0	B.T. Inhibin, beta A
LEPROTL1	BT025351.1	2	100	1e-101	B.T. Leptin receptor overlapping transcript-like 1
CDKN1B	NM_001100346.1	3	99	3e-146	B.T. Cyclin-dependent kinase inhibitor 1B (p27, Kip1)

^aAccession number of the best match found following nucleotide sequence comparison via BLAST search in GenBank.Freq., Frequency of cDNA clone identification from yeast two-hybrid prey library; Ident. (%), Identity: represents homology estimates ofbovine prey cDNA fragments with nucleotide sequences in GenBank database; B.T., Bos taurus
Sequences were analyzed for identity and resulted in candidate proteins potentially interacting with JAK3
Plasmids were purified from true positive yeast colonies, amplified by PCR and sequenced

ISSN: 1757-2215