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Table 3 Antibodies used in the expression analyses of Bos taurus proteins by Western blot

From: Differential regulation of Janus kinase 3 (JAK3) in bovine preovulatory follicles and identification of JAK3 interacting proteins in granulosa cells

Target proteins

Antibodies

Sources

MW (kDa) Conc.

Beta Actin

Chicken IgY anti-Beta actin antibody

Immune Biosolutions (Cat. # Y00005-002)

42

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JAK3

Goat polyclonal anti-JAK3

Santa Cruz Biotechnology (Cat. # sc-1080)

125

0.4 μg/ml

STAT3

Rabbit monoclonal anti-STAT3

Abcam (Cat. # ab32500)

92

0.069 μg/ml

pSTAT3

Rabbit monoclonal anti-pSTAT3

Abcam (Cat. # ab32143)

98

1 μg/ml

STAT5

Rabbit polyclonal anti-STAT5

Abcam (Cat. # ab68465)

90

0.5 μg/ml

pSTAT5

Rabbit polyclonal anti-pSTAT5

Abcam (Cat. # ab97734)

90

1 μg/ml

LEPROTL1

Rabbit polyclonal anti-LEPROTL1

Abcam (Cat. # ab139564)

14

1 μg/ml

INHBA

Rabbit polyclonal anti-INHBA

Aviva Systems Biology (# ARP54663_P050)

44

0.5 μg/ml

CDKN1B

Goat polyclonal anti-CDKN1B/KIP1

Aviva Systems Biology (# OAEB01630)

26

0.1 μg/ml

  1. Abbreviations: pSTAT, phospho STAT, MW molecular weight of target proteins analyzed, Conc., antibody concentrations used for immunoblotting