Fig. 2
In vitro 4 h cytotoxicity of oHSV towards human ovarian cancer cell lines. 48 h after M032 was added to the incubating murine cell lines, Alamar Blue dye was added to each well. 4 and 8 h after the Alamar Blue dye was added to the wells, the mean optical density for each well was calculated and used to construct a dose response plot and the number of viable cells was calculated utilizing regression analysis. A representative figure at 4 h demonstrates the cytotoxicity of M032 towards the human ovarian cancer cell lines (a), and the mean number of PFU/TD50 at 4 and 8 h is demonstrated (b)