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Fig. 5 | Journal of Ovarian Research

Fig. 5

From: MiR-1307 promotes ovarian cancer cell chemoresistance by targeting the ING5 expression

Fig. 5

The changed apoptosis or chemoresistance following miR-1307 loss was rescued by ING5 down-regulation. a The miR-1307 expression by using QRT-PCR in A2780 or SKOV3 cells in the differently treated groups: The miR-1307 expression was decreased in the ASO-1307 following the loss of miR-1307 compared to the ASO con group (P < 0.05, respectively). Besides, the decreased miR-1307 expression was not statistically different between the ING5 siRNA + miR-1307 ASO group or in the siRNA con + miR-1307 ASO group following the loss of miR-1307 in A2780 or SKOV3 cells (P > 0.05, respectively). b The ING5 protein by using western blot in A2780 and SKOV3 cells in the differently treated groups: The ING5 expression was increased in the ASO-1307 or in the siRNA con + miR-1307 ASO group following the loss of miR-1307 in A2780 or SKOV3 cells (P < 0.05, respectively). Meanwhile, the increased ING5 expression was not increased by adding ING5 siRNA following the loss of miR-1307 in A2780 or SKOV3 cells (P < 0.05, respectively). c The results of apoptosis rate in the different groups in A2780 or SKOV3 cells: The results of annexin V-FITC immunofluorescence assay showed that the apoptosis rate increased in the ASO-1307 or in the siRNA con + miR-1307 ASO group following the loss of miR-1307 in A2780 or SKOV3 cells (P < 0.05, respectively). However, the increased cells apoptosis rate following the loss of miR-1307 could be rescued (not increased) by down-regulation of ING5 in A2780 or SKOV3 cells (P < 0.05, respectively). d The results of chemoresistance profiling in the different groups in A2780 and SKOV3 cells: The chemoresistance was decreased in the ASO-1307 or in the siRNA con + miR-1307 ASO group following the loss of miR-1307 (P < 0.01, respectively). However, the decreased chemoresistance following the loss of miR-1307 could be rescued (not increased) by down-regulation of ING5 in A2780 or SKOV3 cells (P < 0.05, respectively). (*P < 0.05)

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