Fig. 1

Plasma-derived anti-glycan antibodies of IgG and IgM isotype bind to polymeric presented Globo H glyco-clusters differentiating healthy controls from ovarian cancer comparable to CA125. a Schematic presentation of the coupling procedure of end-biotinylated glycoconjugates to streptavidin containing beads. b Chemical structure of Globo H conjugated to biotin. c Human AGA (log median fluorescence intensity for IgG and IgM separately) binding to positive (alpha-rhamnose) and negative (aminoglucitol) control. (d & e) Box-and-whisker plot showing distribution of AGA levels directed to Globo H in the blood plasma of serous ovarian cancer (SOC) patients and control group. f ROC curve for both antibody classes (IgG and IgM) together and with CA125 together. Individual CA125 levels are displayed