Fig. 2

Anti-Globo H antibodies bind ovarian cancer cell lines determined to express naturally synthesized Globo H on the cell surface membrane. a Flow cytometry results showing expression of Globo H in selected breast and ovarian cancer cell lines. Representative histograms for cell lines HOSE17.1, FT237, OVCAR3, BG1, MCF10A and MCF7. The percentages of positively stained cells were displayed and are a representative of five independent experiments. Bar chart summarizing five independent experiments on all cell lines tested showing the mean and standard deviation for each cell line. b SGA and FACS show high correlation for binding of plasma-derived anti-Globo H antibodies to Globo H-coupled microbeads (SGA) and Globo-H positive cells (FACS, here MCF7 cells); plasma samples 1–14, dilutions 1/40 (SGA) and 1/200 (FACS). MFI values were log-transformed. c Flow cytometry data represented as histogram for negative control (light grey) and positively stained sample (dark grey). The value provided in each histogram refers to the percentage of positively stained cells