Fig. 7

Morphological changes in the ovarian surface epithelium (OSE) of patients with serous ovarian carcinoma: epithelial cells divided and some round cells (arrows) were separating from the ovarian surface epithelium (a, b). Separated round cells had large nuclei filling almost the entire cell volumes (c, d). Yellow-brown nuclear staining for NANOG in separated round cells, which nicely conformed to blue HE nuclear staining (c, d). Separated round cells (circled) were forming cell clusters (e, f). Some round cells stayed connected by cytoplasmic bridges (arrows) and formed cell clusters (e, f). (Inverted microscope, magnifications 100x and 200x). Legend: yellow to brown-NANOG positivity and blue-nuclei after HE staining. Red Bar: 10 μm for c, d and 100 μm for a, b, e, f