Fig. 4

In vitro regulation of HOAC migration and invasion by Dasatinib, Gefitinib or a combination of both drugs. The Dasatinib and Gefitinib combination suppresses HOAC migration and invasion. HOAC were seeded in Transwell migration inserts in serum-free medium (a) or on a mix of matrigel matrix and serum-free medium (b). After 4 h, complete medium was added to the lower compartment and cells were treated with Dasatinib, Gefitinib (0.1 × IC50 of each cell line after 72 h of treatment) or an equieffective combination of Dasatinib and Gefitinib (Da + Ge) (0.1 × IC50 of each drug alone). Twenty-four hour later, migrating cells or invading cells were counted (Mean +/− SEM, ** = p < 0.01, *** = p < 0.001 n ≥ 3)