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Fig. 2 | Journal of Ovarian Research

Fig. 2

From: Suppressing miR-199a-3p by promoter methylation contributes to tumor aggressiveness and cisplatin resistance of ovarian cancer through promoting DDR1 expression

Fig. 2

The mRNA of DDR1 is a target of miR-199a-3p in ovarian cancer cells. a and b The expression levels of miR-199a-3p (a) and DDR1 (b) in two ovarian cancer cell lines, SKOV3 and HO-8910, and a normal ovarian cell line, IOSE386. *P < 0.05. c The putative human DDR1–3’UTR fragment containing a wild or mutant miR-199a-3p binding site was insert into the luciferase report vector pmirGLO downstream. d and e The DDR1 mRNA (d)and protein (e) levels after the transfection with miR-199a-3p mimics or negative control in SKOV3 cells. *P < 0.05. f and g The DDR1 mRNA (f)and protein (g) levels after the transfection with an anti-miRNA inhibitor specific for miR-199a-3p or negative control in IOSE386 cells. *P < 0.05. h and iLuciferase activity of DDR1 promoter after the indicated transfection in SKOV3 (h) or IOSE386 (i) cells. *P < 0.05. j Luciferase assay in the SKOV3 cells co-transfected with DDR1–3’UTRconstructs containing a wild or mutantmiR-199a-3p binding site as well as miR-199a-3p mimics or scrambled oligonucleotides as the negative control. *P < 0.05

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