Fig. 1

The expression patterns of miR-130b in (a) mature (MO) and immature (IMO) oocytes, (b) immature (IMCC) cumulus, mature (MCC) cumulus and granulosa (GC) cells, (c) preimplantation stage embryos. The expression level of IMO was used as a reference sample to calculate the fold changes between IMO and MO, whereas the expression level of IMCC was used as a reference sample to calculate the fold changes between IMCC, MCC and GC. The expression level of zygotes was used as a reference sample to calculate the fold change expression of miR-130b between preimplantation stage embryos. Bars with different letters are statistically significant (p < 0.05). The bars graphs indicate the mean ± standard deviation (SD) from three independent biological samples of MO, IMO IMCC, MCC, zygotes; 2-cell, 4-cell, and 8-cell stage embryos; morula; and blastocysts