Fig. 2

In situ localization of miR-130b in ovarian sections using 3′-digoxigenin labeled locked nucleic acid (LNA) microRNA probe. The signal intensity of miR-130b in primordial (a), primary follicle (b), secondary follicle (c) and antral follicles (d). The signal intensity of the U6 miRNA (positive control) is indicated in lanes (e) (antral follicle) and (f) (secondary follicle); whereas the signal intensity of the scramble miRNA probe (negative control) is indicated in panel (g) (antral follicle). The red color indicates the expression level of miR-130b, U6 or scramble miRNA probe while the blue color indicates the nuclear staining using 4′,6-diamidino-2-phenylindole (DAPI)