Fig. 7
The effect of miR-130b overexpression or inhibition on cumulus and granulosa cell survival and proliferation. The total number of live cumulus (a) and granulosa (b) cells 24 and 48 h post miR-130b precursor, miR-130b inhibitor, scramble miRNA transfection or untransfected cell groups. The initial plating density (7.5 × 104 cells/ml) was the same in each treatment group before transfection. Direct cell count was performed using trypanblue. The proliferation rate of cumulus (c) and granulosa (d) cells post miR-130b precursor, miR-130b inhibitor, scramble miRNA transfection and untransfected cell groups determined using MTT assay. Results represent the mean ± standard deviation (SD) of three independent replicates. Significant differences (p < 0.05) are indicated by star (*) symbol