Fig. 8

The effect of miR-130b overexpression or inhibition on cumulus cell glycolytic activity (a) and cholesterol biosynthesis (b). The concentration of lactate which is the end product of glycolysis was determined in the cells, while the cholesterol concentration was determined both in the cells and culture media. RFU: Relative fluorescence units. Bars (mean ± standard deviation) with different letters are statistically significant (p < 0.05). The analysis was performed from three independent in vitro cumulus cell culture runs and the cumulus cells were obtained from the cumulus oocyte complexes (COCs) collected from ovaries obtained from slaughterhouse in different days