MicroRNA-130b is involved in bovine granulosa and cumulus cells function, oocyte maturation and blastocyst formation

Table 3 The cleavage, morula and blastocyst rates (mean ± SD) in zygotes injected with miR-130b precursor, miR-130b inhibitor or scramble miRNA and uninjected group

Treatment groups	No. zygotes	First cleavage 48 hpi (%)	Morula rate (%)	Blastocyst rate (%)
miR-130b precursor	490	77.1 ± 5.1	32.7 ± 3.8^a	28.1 ± 2.2^a
miR-130b inhibitor	451	76.1 ± 0.9	23.2 ± 2.5^b	21.3 ± 1.9^b
Scramble miRNA	454	79.9 ± 1.2	27.3 ± 3.8^ab	25.9 ± 3.1^a
Uninjected control	326	79.4 ± 5.1	32.7 ± 2.4^a	27.2 ± 2.6^a

Different letters of superscripts in the same column indicate significant difference (P ≤ 0.05) between treatment groups. Hpi hours post insemination (fertilization), SD standard deviation

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