Fig. 2

MFC gating strategy applied to all ovarian cell suspensions. The first morphological gate is used for debris exclusion using SSC and FSC light scatter (Morphology). Then, an additional gating was performed using SYTO 13 to set nucleated cells (SYTO13⁺) and 7-AAD⁻/SYTO13⁺combination to identify nucleated viable cells. The populations in bold were used to assess cell viability after ovarian tissue dissociation. CD45 gating was used to exclude CD45⁺/CD3⁺ normal T lymphocytes and to select CD45^low population for further functional ovarian tissue qualification or minimal residual disease detection