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Table 1 Cell yield and viability obtained by multicolor flow cytometry analysis after dissociation of reference ovarian tissue and tissue for ovarian tissue cryopreservation

From: Validation of an automated technique for ovarian cortex dissociation: isolation of viable ovarian cells and their qualification by multicolor flow cytometry

Parameters Yield (living ovarian cells/100 mg of tissue) Viability (percentage)
Tissue origin Reference ovarian tissue Tissue from OTC Reference ovarian tissue Tissue from OTC
Fresh or frozen-thawed Fresh Frozen-thawed Fresh Frozen-thawed Fresh Frozen-thawed Fresh Frozen-thawed
n 20 40 5 13 20 40 5 13
Mean 1.56 × 106 1.59 × 106 3.31 × 106 1.09 × 106 38.2 27.0 42.4 16.9
Standard deviation 0.94 × 106 1.35 × 106 2.41 × 106 0.64 × 106 18.2 15.4 21.4 14.3
Minimum 0.34 × 106 0.20 × 106 0.25 × 106 0.81 × 105 9.0 2.0 17.0 1.0
Maximum 4.28 × 106 6.85 × 106 6.22 × 106 2.33 × 106 73.0 67.0 67.0 51.0