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Table 1 Cell yield and viability obtained by multicolor flow cytometry analysis after dissociation of reference ovarian tissue and tissue for ovarian tissue cryopreservation

From: Validation of an automated technique for ovarian cortex dissociation: isolation of viable ovarian cells and their qualification by multicolor flow cytometry

Parameters

Yield (living ovarian cells/100 mg of tissue)

Viability (percentage)

Tissue origin

Reference ovarian tissue

Tissue from OTC

Reference ovarian tissue

Tissue from OTC

Fresh or frozen-thawed

Fresh

Frozen-thawed

Fresh

Frozen-thawed

Fresh

Frozen-thawed

Fresh

Frozen-thawed

n

20

40

5

13

20

40

5

13

Mean

1.56 × 106

1.59 × 106

3.31 × 106

1.09 × 106

38.2

27.0

42.4

16.9

Standard deviation

0.94 × 106

1.35 × 106

2.41 × 106

0.64 × 106

18.2

15.4

21.4

14.3

Minimum

0.34 × 106

0.20 × 106

0.25 × 106

0.81 × 105

9.0

2.0

17.0

1.0

Maximum

4.28 × 106

6.85 × 106

6.22 × 106

2.33 × 106

73.0

67.0

67.0

51.0