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Fig. 2 | Journal of Ovarian Research

Fig. 2

From: Low oxygen tension increases mitochondrial membrane potential and enhances expression of antioxidant genes and implantation protein of mouse blastocyst cultured in vitro

Fig. 2

Immunofluorescence staining images showed that a HIF-2α, b MnSOD and c LIFR protein expression levels were all higher in embryos cultured in 3% O2 than in those cultured in 20% O2. Secondary antibody conjugated with Alexa flour 568-labeled HIF-2α (b, e), LIFR (n, q), and Alexa 488-labeled flour MnSOD (h, k) proteins with DAPI staining of nuclei (a, d, g, j, m, p) and merged images (c, f, i, l, o, r). Scale bar 10 or 20 μm

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