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Fig. 3 | Journal of Ovarian Research

Fig. 3

From: Low oxygen tension increases mitochondrial membrane potential and enhances expression of antioxidant genes and implantation protein of mouse blastocyst cultured in vitro

Fig. 3

Both ROS a, b and apoptosis c, d levels were significantly higher in 20% O2 than 3% O2. a Representative images of intracellular ROS levels in 3% and 20% O2 cultured blastocysts after staining with DCHFDA. b Fluorescent intensity of intracellular ROS levels were higher in 20% O2 (n = 29) than in 3% O2 (n = 31). c Apoptotic nuclei (long arrow) identified by TUNEL were increased in mouse blastocysts cultured under 20% O2. Pseudocolor was representation of nuclei with DAPI. d The apoptotic index was higher in blastocysts cultured in 20% O2 (n = 35) than in 3% O2 (n = 31), expressed as the percentage of apoptotic nuclei divided by the total number of nuclei in each blastocyst. Scale bars 250 and 20 μm. (*P < 0.05)

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Journal of Ovarian Research

ISSN: 1757-2215

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