Fig. 4From: Low oxygen tension increases mitochondrial membrane potential and enhances expression of antioxidant genes and implantation protein of mouse blastocyst cultured in vitroBlastocysts cultured under 3% O2 exhibited significantly higher mitochondrial membrane potential than 20% O2. a Mitochondrial membrane potential (ΔΨm) visualized by JC-1-staining in blastocysts cultured under 3% and 20% O2, respectively. b The level of ΔΨm was significantly higher in blastocysts cultured in 3% O2 (n = 22) compared with those cultured in 20% O2 (n = 20) (*P < 0.05). Scale bar 250 μm. The level of ΔΨm was computed by obtaining the ratio of J-aggregate to J-monomer immunofluorescence intensity for each individual blastocyst sectionBack to article page