Low oxygen tension increases mitochondrial membrane potential and enhances expression of antioxidant genes and implantation protein of mouse blastocyst cultured in vitro

Table 1 List of the primer sequences used for real-time RT-PCR experiment

Gene	Primer Sequence (5′–3′)
HIF-1α	Forward:ACCTTCATCGGAAACTCCAAAG Reverse:ACTGTTAGGCTCAGGTGAACT
HIF-2α	Forward:TAAAGCGGCAGCTGGAGTAT Reverse:ACTGGGAGGCATAGCACTGT
GLUT3	Forward:ATGGGGACAACGAAGGTGAC Reverse:GTCTCAGGTGCATTGATGACTC
VEGF	Forward:CTGCCGTCCGATTGAGACC Reverse:CCCCTCCTTGTACCACTGTC
MnSOD	Forward:GCACATTAACGCGCAGATCA Reverse:AGCCTCCAGCAACTCTCCTT
PRDX5	Forward:CGGAAAGAAGCAGGTTGGGA Reverse:CATCTGGCTCCACGTTCAGT
β-actin	Forward:ACGGCCAGGTCATCACTATTG Reverse:CAAGAAGGAAGGCTGGAAAAGA

HIF-1α hypoxia-inducible factors 1α, HIF-2α hypoxia-inducible factors 2α, GLUT3 glucose transporter 3, VEGF vascular endothelial growth factor, MnSOD manganese superoxide dismutase, PRDX5 peroxiredoxin 5

ISSN: 1757-2215