Fig. 2

CY treatment activates phosphorylation of key proteins in the PI3K/Akt/mTOR pathway. a Immunohistochemistry and immunofluorescence reveal the localization of phospho-Akt, phospho-mTOR and phospho-rpS6 within the oocytes of activated small follicles. b Protein analysis demonstrates an significant increase in the phosphorylated rpS6(*P = 0.025)and slightly increased phosphorylation level of Akt and mTOR. β-actin was used as a loading control. The bar graph shows the results from a single experiment out of the three repeated experiments with similar results; Four ovaries were pooled per result, with a total number of animals of 24