Fig. 3

CY-induced follicle loss and decrease in AMH concentration were reversed by Rapa co-treatment. a Differential follicle counts on ovaries from 8-week-old female BALB/c mice one week after CY treatment at varying doses with or without Rapa co-administration (total n = 12). Data were presented as means ± SEM. *P < 0.05 for the comparison on the same follicle type between CY-treated group and PBS control group; #P < 0.05 for the comparison on the same follicle type between 150 mg/kg with other groups; &P < 0.05 for the comparison between −Rapa group and +Rapa group. (A3) Ratio of growing follicles versus dormant follicles in ovaries from CY-treated mice at varying doses (75, 100, and 150 mg/kg) with or without Rapa co-administration. b AMH plasma concentration measured after single injection with varying CY dose (75, 100, 150 mg/kg) and 4-week treatment with 75mg/kg, as well as PBS alone, Rapa alone (5 mg/kg on alternative days), or CY (150 mg/kg) with or without Rapa (5 mg/kg) on alternative days starting 1 week before CY treatment. Samples were collected 1 week after the final CY administration (Total n = 28). *P < 0.05 for the comparison on the same follicle type between CY-treated group and PBS control group