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Fig. 2 | Journal of Ovarian Research

Fig. 2

From: Collagen triple helix repeat containing 1 (CTHRC1) activates Integrin β3/FAK signaling and promotes metastasis in ovarian cancer

Fig. 2

CTHRC1 activates the integrin β3/FAK signaling in SKOV3 cells. a The protein levels of integrin β3, p-FAK (Tyr397) and FAK in response to CTHRC1 knockdown and overexpression in SKOV3 cells were evaluated by Western blotting (β-actin was used for normalization). b Co-immunoprecipitation of integrin β3 and CTHRC1 in SKOV3 cells revealed that the endogenous CTHRC1 of SKOV3 cells was immunoprecipitated by integrin β3 antibody. c SKOV3-CTHRC1 and SKOV3 cells (control) were treated with MAB 1957 (anti-integrin β3 antibody) and PF-228 (inhibitor of FAK Tyr397 phosphorylation) for 24 h. PF-228 notably restrained the CTHRC1-induced phosphorylation of FAK (Tyr397); nevertheless, the level of integrin β3 protein wasn’t affected by MAB 1957. There was not any statistically significant changes of expression level of CTHRC1, integrin β3, p-FAK and FAK in SKOV3 cells after MAB 1957 and PF-228 treatment. d CTHRC1-induced cell migration and invasion was restrained by MAB 1957 and PF-228 (*P < 0.05, **P < 0.01)

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