Fig. 3
Pathological observation of EOCs with TIC II. H&E staining of (grade 2) EOCs demonstrated incomplete gland lumens, and tumor cells had presented a derangement distribution, and heterotypic cells with partially dark-stained nuclei have been observed (a). Brown microvessels with CD31 staining were observed with IHC method (b). IHC staining showed VEGF-positive expression displayed brownish yellow granules in the cytoplasm and intercellular spaces (c). IHC staining displayed yellow or brown granules appeared in Ki67-positive cell nuclei (d). (×200)