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Fig. 3 | Journal of Ovarian Research

Fig. 3

From: Macrophage colony-stimulating factor (M-CSF) is an intermediate in the process of luteinizing hormone-induced decrease in natriuretic peptide receptor 2 (NPR2) and resumption of oocyte meiosis

Fig. 3

Gonadotropin control of M-CSF, M-CSF-R and NPR2 expression in ovaries in vivo. a Localization of M-CSF, M-CSF-R, and NPR2 in ovarian follicles shown with immunohistochemistry. Red box represents the time point 48 h after eCG treatment. Scale bars, 100 μm. b Mean optical density of ovarian slides. *, P < .05; ▲, P < .01 compared with corresponding 48-h value in the red box. Bars show the mean ± SEM of three independent slides. c Expression of M-CSF, M-CSF-R, and NPR2 mRNA by quantitative RT-PCR. The value in the control (0-h eCG) was set at a value of 1, and levels of expression in other samples are demonstrated relative to the control. *, P < .05 compared with the corresponding control. Bars show the mean ± SEM of three independent experiments

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