Table 1 Oligonucleotide sequences
From: Docosahexaenoic acid mechanisms of action on the bovine oocyte-cumulus complex
abbrev. | accession number | gene | Forward primer | Reverse primer | bp | E % |
|---|---|---|---|---|---|---|
AHCY | adenosylhomocysteinase | TGGACCCACCCAGACAAGTA | CAAAGGGCAGGTCTGGTTCT | 202 | 95.8 | |
DHRS1 | dehydrogenase/reductase (SDR family) member 1 | GGGGGCTGCACTATCTCTTC | AATAATCCTGGACAGGGCGG | 366 | 87.1 | |
EEF1G | eukaryotic translation elongation factor 1 gamma | CCAAGGATCCCTTTGCCCAT | GGAGAGCGTATCCTCGTTGG | 86 | 93 | |
EIF2S1 | eukaryotic translation initiation factor 2, subunit 1 alpha | GTGGGACCTTGTTGTGGGAT | AGCTGACATAAGCGCCCATT | 144 | 97.1 | |
SLC2A1 = GLUT1 | NM_174602 | solute carrier family 2 (facilitated glucose transporter), member 1 | CTGATCCTGGGTCGCTTCAT | ACGTACATGGGCACAAAACCA | 68 | 91.4 |
GAPDH | NM_001034034 | Glyceraldehyde 3 phosphate dehydrogenase | TTCAACGGCACAGTCAAGG | ACATACTCAGCACCAGCATCAC | 119 | 98 |
GPX1 | NM_174076 | glutathione peroxidase 1 | GCAACCAGTTTGGGCATCA | CTCGCACTTTTCGAAGAGCATA | 116 | 87.3 |
GPX4 | NM_174770 | glutathione peroxidase 4 | CGATACGCCGAGTGTGGTTTAC | ACAGCCGTTCTTGTCAATGAGG | 261 | 89.8 |
GSN | gelsolin | GGACCAGGTCTTTGTCTGGG | GATTAGCTGGGTCCGTCTCG | 101 | 92.1 | |
FFAR4 | XM_865266 | free fatty acid receptor 4 | TTTCACTGCTGCTTCTTCGC | TTTCTGAGAGCCGGTACCCT | 197 | 87.9 |
FFAR1 | XM_002694932 | free fatty acid receptor 1 | GCCATCGTTCTCTGTCACCT | CCGGAGAGCCATTGATTGGT | 112 | 87.5 |
Met_SRP | Metazoan signal recognition particle RNA | CGCCTGTAGTCCCAGCTACT | ATCAGCACGGGAGTTTTGAC | 211 | 90.1 | |
MTMR3 | myotubularin related protein 3 | CTGCGAAAAGCTGCTAACCG | CCTGAGACACAACTTGCCCT | 119 | 90.8 | |
RPL19 | BC102223 | Ribosomal protein L19 | AATCGCCAATGCCAACTC | CCCTTTCGCTTACCTATACC | 156 | 97.3 |
RPS9 | BC148016 | Ribosomic protein S9 | GGAGACCCTTCGAGAAGTCC | GGGCATTACCTTCGAACAGA | 180 | 94.2 |
SIRT2 | sirtuin 2 | CTGGCCAGACTGACCCTTTC | CTTCCATCCAAGGAGGTCGG | 146 | 91.3 | |
SNORA16 | Small nucleolar RNA SNORA16B/SNORA16A family | CGTGGCCCTTATCGAAGCTG | GCGACCGTCAAGGAAAACTG | 87 | 90.6 | |
SNORA17 | Small nucleolar RNA SNORA17 | AGAGGAAAGAGGCTCGGTCT | AGTGTCAGACTATCAATCATCCAGA | 71 | 82.8 | |
SNOU6_53 | Small nucleolar RNA U6–53/MBII-28 | GTTGCCATGCTAATACTGAGCC | TGTTAAACTCACTGGCACCCA | 70 | 94.3 | |
U1 | U1 spliceosomal RNA | CACGAAGGTGGTTTTCCCTA | GCCCCCACTACCACAAGTTA | 114 | 110 | |
VCP | valosin containing protein | TGCTTGCAAGTTGGCCATTC | GCAAAGCGCATAGCTTCCTC | 150 | 83.5 |