Fig. 2
Effect of MFR on the number of follicles per section of ovarian tissue. a Total number of follicles: MFR significantly affects the total number of follicles in ovaries from FR1 females at 4 weeks (P = 0.0006) and 8 weeks (P = 0.0485), and from FR2 females at 8 weeks (P = 0.0013). The total number of follicles from FR2 at week 4 is higher vs. FR females (P = 0.0020), whereas it is lower in FR2 females at week 8 compared to FR females (P = 0.0074). b Primordial follicles: significant effect of MFR on the number of primordial follicles in FR1 and FR2 females at week 4 when compared to control (P = 0.0014 and P < 0.0001, respectively) and in FR2 females at week 8 (P = 0.0002). The total number of primary follicles from FR2 at both week 4 and 8 is significantly lower when compared to FR1 females (P = 0.0020 and P = 0.003, respectively). c Primary follicles are significantly higher in number in ovaries of 4-week-old females in both FR1 and FR2 vs. controls (P = 0.034 and P = 0.003). At week 8, FR1 females have significantly higher number of primary follicles vs. control (P = 0.0072), whereas FR2 females have significantly lower number of primary follicles vs. FR1 (P = 0.0014). d Secondary follicles: FR1 females at week 4 have significantly lower number of secondary follicles vs. control (P = 0.0002) and vs. FR2 (P = 0.001), whereas at week 8 FR2 have significantly higher number of secondary follicles vs. FR1 females (P = 0.0002). e Antral follicles: MFR has the same effect as in secondary follicles, except for a significant increase in FR2 females vs. control at week 8 (P = 0.008). f MOFs: The number of MOFs is significantly higher in ovaries from FR and FR2 females vs. control at week 4 (P = 0.0044 and P = 0.006, respectively); it is also significantly higher in FR1 females at week 8 (P = 0.0013), and again significantly lower in ovaries from FR2 females vs. control (P = 0.0075). FR2 females at week 8 have significantly lower number of MOFs when compared to FR1 females (P < 0.0001). All results are given as mean ± SD; P < 0.05, *FR1 vs. controls (C) and FR2 vs. controls, * with error bars: FR1 vs. FR2