Fig. 1
From: DHEA-induced ovarian hyperfibrosis is mediated by TGF-β signaling pathway
DHEA-induced PCOS-like rat and ovarian fibrosis. Ovarian and follicular morphology of dehydroepiandrosterone (DHEA)-exposed rats and controls, assessed by H&E staining. Normal follicular morphology, thicker granular cell layer and higher number of corpus luteum was observed in blank and oil-treated group compared to Oil + DHEA-treated group. (A-C) magnification × 50; (D-F) magnification × 100. The detection of ovarian fibrosis. Collagen of ovaries slices was stained by Sirius red to observe the effects of DHEA on rat ovarian fibrosis. (a) Blank; (b) Oil-treated; (c) Oil + DHEA-treated. ×100 (d-f) Higher magnification views corresponding to A-C. × 400. Rat ovarian collagen IV detected by immunohistochemical analysis. (g) Oil group; (h) Oil + DHEA group. ×100. “→” is directed in the direction of fibrosis. “▲” is directed in the direction of collagen IV; C, corpus luteum; F, follicle; A, albuginea