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Fig. 5 | Journal of Ovarian Research

Fig. 5

From: A protocol to isolate and qualify purified human preantral follicles in cases of acute leukemia, for future clinical applications

Fig. 5

Cell suspensions observed under an inverted micromanipulation microscope. a Leukemic cell suspension obtained after thawing, corresponding to positive controls; b Ovarian cell suspension, containing follicles and leukemic cells, without wash; c Ovarian cell suspension obtained after 3 washes of isolated follicles; d An example of ovarian cell suspension obtained after 3 washes of isolated follicles, with two leukemic cells visible (black arrows); e The same ovarian cell suspension obtained after 3 washes under inverted fluorescence microscope, showing the presence of two leukemic cells stained by using a fluorescent cell tracer (white arrows)

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Journal of Ovarian Research

ISSN: 1757-2215

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