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Table 1 Classification of isolated follicles per protocol after enzymatic dissociation of ovarian cortical strips

From: A protocol to isolate and qualify purified human preantral follicles in cases of acute leukemia, for future clinical applications

 

Follicles (n)

Primordial (%)

Primary (%)

Secondary (%)

Collagenase IA (200 IU/ml)

319

247 (77.5)

55 (17.2)

17 (5.3)

Collagenase NB6 (200 IU/ml)

362

303 (83.7)

48 (13.3)

11 (3)

Liberase DH (0.28 Wünsch U/ml)

205

154 (75.1)

30 (14.6)

21 (10.3)

  1. % = percentage of each follicular stage out of the total isolated follicles per protocol