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Table 2 Viability of isolated follicles after dissociation of ovarian cortical strips

From: A protocol to isolate and qualify purified human preantral follicles in cases of acute leukemia, for future clinical applications

 

Follicles (n)

V1

V2

V3

V4

Collagenase IA (200 IU/ml)

298

191

85

17

5

Collagenase NB6 (200 IU/ml)

272

194

60

14

4

Liberase DH (0.28 Wünsch U/ml)

198

145

43

10

0

  1. Viability from V1 to V4, using collagenase IA (n = 7 patients), collagenase NB6 (n = 6 patients), and Liberase DH (n = 5 patients), according to the number of dead granulosa cells