A protocol to isolate and qualify purified human preantral follicles in cases of acute leukemia, for future clinical applications

Table 4 Results obtained after analysis by MFC of the isolated follicle suspensions after adding leukemic cells

					MFC
					Number of leukemic cells detected		Results
Patients	Cortical biopsie weight (mg)	Number of isolated follicles	Number of added leukemic cells	Type of leukemia	Before wash	After 3 washes	Before wash	After 3 washes
1	46.2	93	1.10⁶	B-ALL	4889	0	POSITIVE	NEGATIVE
1	46.2	89	1.10⁵	B-ALL	452	4	POSITIVE	NEGATIVE
1	46.2	91	1.10⁴	B-ALL	32	0	POSITIVE	NEGATIVE
2	69.6	4	1.10⁶	B-ALL	7	0	NEGATIVE	NEGATIVE
2	69.6	10	1.10⁵	B-ALL	6	0	NEGATIVE	NEGATIVE
2	69.6	17	1.10⁴	B-ALL	1	0	NEGATIVE	NEGATIVE
3	77	50	1.10⁵	AML	15	3	NEGATIVE	NEGATIVE
3	77	35	1.10⁴	AML	1	0	NEGATIVE	NEGATIVE
3	77	31	1.10³	AML	3	0	NEGATIVE	NEGATIVE
4	70.9	11	1.10⁶	AML	177	4	POSITIVE	NEGATIVE
4	70.9	5	1.10⁵	AML	11	1	NEGATIVE	NEGATIVE
4	70.9	3	1.10⁴	AML	3	0	NEGATIVE	NEGATIVE
5	31.7	47	1.10⁶	AML	3434	22	POSITIVE	POSITIVE
5	31.7	46	1.10⁵	AML	388	3	POSITIVE	NEGATIVE
5	31.7	36	1.10⁴	AML	37	2	POSITIVE	NEGATIVE
6	59.3	88	1.10⁶	AML	3799	6	POSITIVE	NEGATIVE
6	59.3	96	1.10⁵	AML	1265	3	POSITIVE	NEGATIVE
6	59.3	65	1.10⁴	AML	36	1	POSITIVE	NEGATIVE
7	38.6	12	1.10⁶	AML	2069	7	POSITIVE	NEGATIVE
7	38.6	20	1.10⁵	AML	206	2	POSITIVE	NEGATIVE
7	38.6	16	1.10⁴	AML	25	2	POSITIVE	NEGATIVE
8	38.6	63	1.10⁶	AML	1942	1	POSITIVE	NEGATIVE
8	38.6	68	1.10⁵	AML	202	0	POSITIVE	NEGATIVE
8	38.6	72	1.10⁴	AML	15	0	NEGATIVE	NEGATIVE
Total		1068			19,015	61
Mean					792	3
Standard deviation					1402	5
Number of experiments	24

Results obtained before and after three washes of 24 ovarian suspensions containing isolated follicles, in which serial dilutions of leukemic cells (from 10³ to 10⁶) were artificially added

ISSN: 1757-2215