Fig. 1

Immuno-phenotyping studies on stem cells in sheep ovarian surface epithelial (OSE) cells. Immunophenotyping analysis for OCT-4 was carried out on sheep OSE cells (a) using standard calibration beads of size ranging from 2-6 μm gated as P1 (b) sheep OSE cellsin the size range of 2-6 μm size representing P1 population (c) represents unstained cells for FITC gated as P3 population. (d) Immuno-phenotyping analysis for stem cells in the size range of 2-6 μm with (e) 4.4% cells expressing (untagged) OCT-4 antibody labelled with Alexafluor 488 whereas (f) represents percentage of cell population negative for OCT-4 using only Alexafluor 488 without primary antibody serving as negative control