Fig. 12

OCT-4 and FSHR immuno-localization on sheep ovarian sections. a Few cells on OSE were positive for OCT-4 (b-d) cluster of oocytes/oogonia in primordial and primary follicles and ooplasm were positive for OCT-4 whereas (c-e) surrounding granulosa cells and stromal cells were completely negative. Oocytes in large secondary and tertiary follicles in medulla region also showed OCT-4 expression whereas surrounding granulosa cells were negative. f-g Negative control with omission of primary antibody. h-i OSE cells show FSHR expression, (j) cluster of oocytes, (k-m) individual primordial and primary follicles oocytes nucleus and ooplasm both were positive for FSHR including surrounding granulosa cells, stromal cells were completely negative. n-o In medulla region larger oocytes & ooplasm including surrounding granulosa cells were positive for FSHR. p Negative control with omission of primary antibody (q-r) Negative control using peptide blocking of antibody