Fig. 2

Immuno-phenotyping studies for OCT-4 and co-expression of OCT-4 and FSHR. Immuno-phenotyping analysis for OCT-4 and FSHR + OCT-4 was carried out on sheep OSE cells (gating strategy similar to that described in Fig. 1) (a) represents standard calibration beads of size ranging from 2–6 μm gated as P1, (b) sheep OSE cellsin the size range of 2-6 μm size representing P1 population (c, d) unstained cells for both PerCP-Cy5-5A and FITC gated as P5 and P3 populations respectively. (e) Immuno-phenotyping analysis for stem cells in the size range of 2-6 μm with (f) 4% cells expressing PerCP-Cy5-5A tagged OCT-4 antibody and (g) sheep OSE cells in the size range of 2–6 μm size representing P1 population (h) represents unstained cells for both PerCP-Cy5-5A and FITC (i) 4% of cells in the size range of 2–6 μm were OCT-4 positive and 6.5% expressed FSHR of which 1.6% of cells were also positive for OCT-4