Fig. 5

Immunohistochemistry and western blot analysis of TLR2 and TLR4 in serous papillary OC. The immunoreactivity for TLR2 was moderate (arrow) in the P-MAPA (b) compared to a weak immunostaining in OC animals (a). While CIS therapy (c) showed a weak immunoreaction for TLR2, the combination of P-MAPA and CIS exhibited variations from weak to moderate (d) (arrows). TLR4 immunoreactivity was intense (arrow) in the P-MAPA (f) compared to weak reaction in the OC animals (e). In addition, the combination of P-MAPA with CIS promoted an intense immunoreaction to TLR4 (h) compared to CIS therapy (g) (arrows). Bar = 20 μm. Negative controls were used. i Expression profile of the TLR2 and TLR4 in extracts of 50 μg proteins pooled from 5 samples per group. j, k Individual blots were used for densitometric analysis of the TLR2 and TLR4 levels following normalization to the β-actin. Data are expressed as the mean ± SD. ^a P < 0.05 vs. OC; ^b P < 0.05 vs. CIS