Fig. 6

Western blot analysis of MyD88, TRIF, IKK-α, p-IkBα, and NF-kB p65 and fluorescence localization in serous papillary OC. Protein profiles of representative cytosolic (a) and nuclear (c) fractions (50 μg protein) pooled from 5 samples per group. b Individual blots were used for densitometric analysis of the protein levels following normalization to β-actin or Lamin B1. Data are expressed as the mean ± SD. ^* P < 0.05 vs. the OC group. d Merged images of NF-kB p65 immunofluorescence and DAPI nuclear staining in the OC (I), P-MAPA (II), CIS (III), and P-MAPA+CIS (IV) groups (Alexafluor®488, Bar = 10 μm). MyD88: Myeloid differentiation factor 88; TRIF: TLR-associated activator of interferon; IKK-α: inhibitor of NF-kB kinase subunit alpha; p-IkBα: inhibitor of phosphorylated NF-kB subunit alpha; NF-kB p65: NF-kB subunit p65 (RelA)