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Fig. 4 | Journal of Ovarian Research

Fig. 4

From: Sialylation of EGFR by the ST6Gal-I sialyltransferase promotes EGFR activation and resistance to gefitinib-mediated cell death

Fig. 4

EGFR phosphorylation is enhanced in OV4 ovarian cancer cells with ST6Gal-I overexpression. a. To measure levels of α2-6 sialylated EGFR, cell lysates were incubated with SNA-agarose. SNA is a lectin that specifically recognizes α2-6 sialic acids. Sialylated proteins were precipitated and then immunoblotted for EGFR. b. Representative p-EGFR immunoblot of EV or OE cells treated with EGF for 5, 15 and 30 min. c. Densitometric analysis of three independent blots of p-EGFR in EGF-treated OV4 cells, with values normalized to the β-tubulin loading control. d. Representative p-EGFR immunoblot of EV or OE cells to evaluate basal EGFR phosphorylation. e. Densitometric analysis of three independent blots of basal p-EGFR in OV4 cells. *, p < 0.05

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