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Fig. 6 | Journal of Ovarian Research

Fig. 6

From: Sialylation of EGFR by the ST6Gal-I sialyltransferase promotes EGFR activation and resistance to gefitinib-mediated cell death

Fig. 6

Knockdown of ST6Gal-I diminishes EGFR phosphorylation in BxPC3 pancreatic cancer cells. a. Using lentivirus, BxPC3 cells were stably transduced with shRNA for ST6Gal-I, and ST6Gal-I knockdown (KD) was confirmed by immunoblotting. EV or KD cell lysates were precipitated by SNA agarose and immunoblotted for EGFR to detect α2-6 sialylated EGFR. Total cell lysates were immunoblotted for activated EGFR (p-EGFR, pY1068) or total EGFR. b. Densitometric analysis of three independent blots of basal p-EGFR in BxPC3 cells (normalized to β-tubulin). c. Representative p-EGFR immunoblot of EV or KD cells treated with EGF for 5, 15 and 30 min. d. Densitometric analysis of three independent blots of p-EGFR in EGF-treated BxPC3 cells. e. Total cell lysates from EV cells of the OV4, SKOV3 and BxPC3 lines were immunoblotted for ST6Gal-I, p-EGFR (Y1068) or total EGFR. *, p < 0.05

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