Fig. 1

RT real-time PCR analysis of meiosis related gene expression in differentiation cultures exposed to RA or vehicle only for 48 h. a The expression of Stra8 was significantly higher (11.63 ± 7.64 fold) following exposure to 10 μm RA when compared to the vehicle only control. b Similarly, the expression of Sycp3 was significantly higher (1.65 ± 0.42 fold) following exposure to 10 μm RA when compared to the vehicle only control. Conversely, the expression of the meiosis inhibitor gene Nanos2 was significantly lower, 0.32 ± 0.08 fold when compared to the vehicle only contol. * denotes significant difference p < 0.05 using a t-test.