Fig. 5
From: Retinoic acid enhances germ cell differentiation of mouse skin-derived stem cells
The expression of Figla, and Zp1-Zp3 was tested in the differentiations using RT-PCR (a). The gel is loaded with 1 kb marker (M) followed by PCR products from ovary (O), RA treated (RA), and control (c) differentiations repeated for the Figla and Zp1–3 transcripts (a). b Loading controls for the PCR reactions. Hprt was usied as a positive loading control (left panel). Primer only negative controls, loaded left to right 1 kb marker (M), Figla (F), Zp1–3, and Hprt, using water to replace cDNA (right panel). c Representative immunofluorescent images depicting the expression of OCT4 (red) and ZP3 (green) in RA treated (left panel) and control OLCs (right panel). b Scale bars = 30 μm