Fig. 1

Cell cycle distributions in native and paclitaxel resistant cell lines (a) TOV21G, (b) TOV112D and (c) COV504. Using a double thymidine block, native and paclitaxel resistant cells were synchronized and incubated in the presence of DMSO or 25 nM paclitaxel. Cells were collected at 12 hours and the cell cycle distributions within the cell population were analysed by flow cytometry. The cellular response of 3 cell lines was consistent whereby the resistant ovarian cancer cells treated with paclitaxel were able to overcome paclitaxel induced G2/M arrest and progressed through the cell cycle. The percentages shown represent a single experiment; 3 independent experiments were conducted for each cell line