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Fig. 4 | Journal of Ovarian Research

Fig. 4

From: Deregulation of the spindle assembly checkpoint is associated with paclitaxel resistance in ovarian cancer

Fig. 4

The effect of paclitaxel resistance on key regulators of the mitotic spindle checkpoint. Immunoblot analysis of cell cycle proteins BubR1, cyclin B1, CENPE and CENPF isolated from native and resistant cell lines. Individual lanes contained 20-50 μg of total protein; each gel was normalized. The proteins were resolved on 4-20% Mini Protean TGX Precast Gels, transferred to nitrocellulose membranes and probed using antibodies specific for BubR1, cyclin B1, CENPE and CENPF proteins. β-actin served as a loading control. The immunoblots represent a single experiment; 3 independent experiments were conducted

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