Fig. 9
The tumorigenicity of A2780 + 20 GFP (+) cells in vivo. a) The xenografts produced by the A2780 + 20 GFP (+) cells were larger than those produced by the GFP (−) cells; 5 × 104 cells were injected. b-d) AR expression in A2780 + 20 GFP (+) tumors was stronger than that in GFP (−) tumors. Similarly, Nanog expression was increased in A2780 + 20 GFP (+) tumors. e and f) For the in vivo hormone treatment groups, 12 mice (injected with 5 × 103 A2780 + 20 GFP-positive cells) underwent xenografting, but one died before treatment. The tumor volume in the androgen treatment group increased more quickly and was greater than that in the anti-androgen group (P < 0.05). Fluorescence was weak after the anti-androgen treatment compared to that with androgen treatment. Photographs were captured with an in vivo imaging system