Name | Sequences (5′- > 3′) | PAM/ Length | |
---|---|---|---|
Forward | Reverse | ||
CRISPR gRNAs for BMI1 | |||
 gRNA1 | CACCGAACGTGTATTGTTCGTT | AAACGGTAACGAACAATACACGTTC | ACC |
 gRNA2 | CACCGTGGTCTGGTCTTGTGAAC | AAACAGTTCACAAGACCAGACCAC | TGG |
Primers for PCR | |||
 BMI1a | TTGATGCCACAACCATAATAGAAT CTAACACCAATGATTTATCCACTC | AATTACAAACAAGGAATTTCAACA | 494 bp 194 bp |
Primers for real-time PCR | |||
 Caspase3 | GAAATTGTGGAATTGATGCGTGA | CTACAACGATCCCCTCTGAAAAA | 166 bp |
 Bcl-2 | CTAAGGGTATGAAGGACCTGTA | CTCTGGAATCTAAAGGTCGT | 111 bp |
 COL1A1 | GTGCGATGACGTGATCTGTGA | CGGTGGTTTCTTGGTCGGT | 119 bp |
 COL4A1 | GGACTACCTGGAACAAAAGGG | GCCAAGTATCTCACCTGGATCA | 240 bp |
 TNC | TCCCAGTGTTCGGTGGATCT | TTGATGCGATGTGTGAAGACA | 131 bp |
 LAMA3 | TGCTCAACTACCGTTCTGCC | TCCAGTTCTTTTGCGCTTTGT | 181 bp |
 ITGA7 | CAGCGAGTGGACCAGATCC | CCAAAGAGGAGGTAGTGGCTATC | 203 bp |
 ITGB4 | CTCCACCGAGTCAGCCTTC | CGGGTAGTCCTGTGTCCTGTA | 133 bp |
 AKT3 | AATGGACAGAAGCTATCCAGGC | TGATGGGTTGTAGAGGCATCC | 130 bp |
 CREB5 | AAAGACTGCCCAATAACAGCC | AAGCTGGGACAGGACTAGCA | 88 bp |
 PIK3CA | GAAACAAGACGACTTTGTGACCT | CTTCACGGTTGCCTACTGGT | 76 bp |
 PIK3CD | AGCCGGAAGACTACACGCT | GGTCAGGTGAGGGGTCAAC | 122 bp |
 BIRC3 | TTTCCGTGGCTCTTATTCAAACT | GCACAGTGGTAGGAACTTCTCAT | 96 bp |
 GAPDH | ACAACTTTGGTATCGTGGAAGG | GCCATCACGCCACAGTTTC | 101 bp |