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Fig. 4 | Journal of Ovarian Research

Fig. 4

From: MicroRNA-424/503 cluster members regulate bovine granulosa cell proliferation and cell cycle progression by targeting SMAD7 gene through activin signalling pathway

Fig. 4

Overexpression of the miR-424/503 cluster members increased bovine granulosa cell proliferation. Cell proliferation assay of granulosa cells transfected with miR-424/503 cluster mimics and inhibitors and the corresponding negative control (NC) (a). The mRNA (b) and protein (c) expression level of PCNA and STAR in granulosa cells transfected with miR-424/503 cluster mimics, inhibitors or corresponding NC. The protein expression level of SMAD2/3 and phosphorylated SMAD2/3 in granulosa cells transfected with miR-424/503 cluster mimics and inhibitors and the corresponding NC (d). The bar graphs indicate the mean ± SEM (**p < 0.01, ***p < 0.001). Numbers above western blots represent relative protein density

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